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Gibco serum
- Sv30160.03 fetal bovine serum
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Hengyuan bio mainly sells serum of GIBCO, hyclone, biological industries, sigma and other brands, with a variety of serum, including fetal bovine serum, calf serum, horse serum and other animal serum.
Serum quality is stable, low endotoxin, no bacteria, mycoplasma, bacteriophage, virus and other pollution, no external factors, no hormone and so on.
After nearly 1000 cell lines, more than 500 kinds of primary cells, more than 100000 times of cell culture verification, only for you to use at ease. Hengyuan bio has been providing high-quality serum for the majority of drug R & D, vaccine R & D enterprises, hospitals, universities and other scientific research institutions. Welcome to inquire!
Product Name: sv30160.03 fetal bovine serum
2. - 20 ℃, dark and constant temperature refrigerator, avoid repeated freezing and thawing!
The company supplies genuine fetal bovine serum with high quality, first-class service, sincere attitude, insist on winning by quality, be your experimental expert!
The function of fetal bovine serum is as follows
Fetal bovine serum (FBS) is the most widely used natural medium in cell culture. It is rich in nutrients necessary for cell growth. It is often used in vitro culture of animal cells and has very important functions.
1. Provide hormones to maintain the exponential growth of cells, nutrients that are absent or few in the basic medium, and major low molecular nutrients.
2. Provide binding proteins, which can recognize vitamins, lipids, metals and other hormones, and can bind or modulate the activity of substances they bind.
3. In some cases, binding proteins can bind with toxic metals and pyrogens to detoxify.
4. It is the source of factors needed for cell adhesion and spreading on plastic culture medium.
5. Act as pH buffer.
6. Provide protease inhibitors to inactivate the remaining trypsin during cell passage and protect cells from injury.
Operation steps of sv30160.03 fetal bovine serum:
Serum is usually used as an additive for basal growth medium in cell culture. The common serum is fetal bovine serum (FBS), also known as FCS. Fetal bovine serum is collected from farms suitable for human health practices. Sometimes, other bovine serum may be used, such as newborn bovine serum or donor bovine serum. In cell culture, serum is rich in high molecular weight protein, low molecular weight nutrients, hydrophobic carrier protein and other components necessary for cell growth in vitro, such as hormones and adhesion factors. At the same time, serum can increase the buffering capacity of medium or neutralize toxic components, and play a role in protecting cells. Whether to add serum in the process of cell culture depends on the chemical composition of the basic medium, the type of cultured cells and the culture system.
1. Collection
During the production of fetal bovine serum, whole blood was collected in sterile disposable plastic bags, separated after coagulation, collected and frozen for storage. Control of the initial collection of fetal bovine serum is the key factor to control the quality of final serum products. Production can only be allowed if the initial raw materials meet our specifications.
2. Serum material
There are two fetal bovine serum standards in the global market: USDA and European standards. According to the standard of USDA, the original serum must be collected from countries without BSE and FMD before it can be freely imported into any country for safe production. Moreover, only when researchers use sera that meet this standard can they be allowed to send their cells and cell products to other countries with strict import terms for cooperation and exchange.
3. Treatment process
Several batches of frozen serum were selected for thawing, and the contents of endotoxin and heme were tested. Only when the standard was met, the frozen serum could be fully mixed for further processing and filtration. Nqbb processes fetal bovine serum by prefiltration and membrane filtration. The final filtration step is to use 100 nm membrane for three times. At the end of filtration, the serum was separated by aseptic process, and the whole process effectively ensured the sterility of the final product. The air cleanliness of serum production is 100. All subpackage benches are filtered by high efficiency particulate air filters, and maintain a positive pressure subpackage environment to monitor the total number of particles, suspended particles and pressure. After subpackage, the final product was frozen at - 20 ℃ and isolated until all quality control tests were completed.
4. FBS quality control
Each batch of fetal bovine serum was confirmed to meet the standards, and recorded in the quality inspection report in written form.
5. Quality assurance
The production process of fetal bovine serum was carried out under a strictly controlled condition. Ultraviolet sterilization, filtration, sterilization and sub packaging are the key production links, which can ensure the quality of serum. The data related to serum production is recorded as a special file, from the initial collection of raw materials to the storage of finished products, the whole production process, as well as the quality control test and results are recorded, and the source documents can be traced under monitoring conditions. The production process of fetal bovine serum follows the European Parliament standard for in vitro diagnosis.
(2) Heat inactivation refers to the complete thawed serum heated at 56 ℃ for 30 minutes. During the heating process, it must be shaken regularly and evenly. The purpose of this heat treatment is to inactivate the complement components in the serum. Unless necessary, this heat treatment is generally not recommended, because heat treatment will cause a significant increase in serum sediment and affect the quality of the serum, Smooth muscle cells contract, mast cells and platelets release histamine, enhance phagocytosis, promote chemotaxis and activation of lymphocytes and macrophages
(3) The serum in the refrigerator from - 20 ℃ to - 70 ℃ should be dissolved in the refrigerator at 4 ℃ for 1 day. Then, it should be moved to room temperature and then sub packed. During the dissolution process, it should be shaken gently and evenly (be careful not to cause bubbles) to make the temperature and composition uniform and reduce the occurrence of precipitation. Do not thaw the serum directly from - 20 ℃ to 37 ℃, Because the temperature changes too much, it is easy to cause protein agglutination and precipitation
(4) Generally, the serum provided by the manufacturer is sterile and does not need to be filtered. If the serum is found to have suspended substance, the serum can be added into the culture medium and filtered together. Do not filter the serum directly
(5) Precipitate floccules in serum: mainly caused by lipoprotein denaturation in serum and fibrin in serum after thawing. These floccules will not affect the quality of serum itself. They can be removed by centrifugation at 3000rpm for 5 minutes or without treatment, In general, the small black spots will not affect cell growth, but if the quality of serum is suspected, it should be stopped immediately and replaced with another batch of serum
(6) Do not store the serum at 37 ℃ for too long, otherwise the serum will become turbid, and the effective components in the serum will be broken, which will affect the quality of serum storage / transportation: - 5 ° C to - 20 ° C.
Product No.: sv30160.03
Product brand: hyclone
Unit specification: 500ml
Blood source: South American origin
Scope of application: for a wide range of cell types, mainly some common cell lines, low levels of endotoxin and hemoglobin in serum, endotoxin level: ≤ 10 EU / ml, hemoglobin level: ≤ 25 mg / dl, triple filtration 0.1 μ M. Warm tips: the product is for research use only in non clinical unit.Transportation, preservation and points for attention:
1. Dry ice is transported all the way to ensure your use!2. - 20 ℃, dark and constant temperature refrigerator, avoid repeated freezing and thawing!
The company supplies genuine fetal bovine serum with high quality, first-class service, sincere attitude, insist on winning by quality, be your experimental expert!
The function of fetal bovine serum is as follows
Fetal bovine serum (FBS) is the most widely used natural medium in cell culture. It is rich in nutrients necessary for cell growth. It is often used in vitro culture of animal cells and has very important functions.
1. Provide hormones to maintain the exponential growth of cells, nutrients that are absent or few in the basic medium, and major low molecular nutrients.
2. Provide binding proteins, which can recognize vitamins, lipids, metals and other hormones, and can bind or modulate the activity of substances they bind.
3. In some cases, binding proteins can bind with toxic metals and pyrogens to detoxify.
4. It is the source of factors needed for cell adhesion and spreading on plastic culture medium.
5. Act as pH buffer.
6. Provide protease inhibitors to inactivate the remaining trypsin during cell passage and protect cells from injury.
Operation steps of sv30160.03 fetal bovine serum:
Serum is usually used as an additive for basal growth medium in cell culture. The common serum is fetal bovine serum (FBS), also known as FCS. Fetal bovine serum is collected from farms suitable for human health practices. Sometimes, other bovine serum may be used, such as newborn bovine serum or donor bovine serum. In cell culture, serum is rich in high molecular weight protein, low molecular weight nutrients, hydrophobic carrier protein and other components necessary for cell growth in vitro, such as hormones and adhesion factors. At the same time, serum can increase the buffering capacity of medium or neutralize toxic components, and play a role in protecting cells. Whether to add serum in the process of cell culture depends on the chemical composition of the basic medium, the type of cultured cells and the culture system.
1. Collection
During the production of fetal bovine serum, whole blood was collected in sterile disposable plastic bags, separated after coagulation, collected and frozen for storage. Control of the initial collection of fetal bovine serum is the key factor to control the quality of final serum products. Production can only be allowed if the initial raw materials meet our specifications.
2. Serum material
There are two fetal bovine serum standards in the global market: USDA and European standards. According to the standard of USDA, the original serum must be collected from countries without BSE and FMD before it can be freely imported into any country for safe production. Moreover, only when researchers use sera that meet this standard can they be allowed to send their cells and cell products to other countries with strict import terms for cooperation and exchange.
3. Treatment process
Several batches of frozen serum were selected for thawing, and the contents of endotoxin and heme were tested. Only when the standard was met, the frozen serum could be fully mixed for further processing and filtration. Nqbb processes fetal bovine serum by prefiltration and membrane filtration. The final filtration step is to use 100 nm membrane for three times. At the end of filtration, the serum was separated by aseptic process, and the whole process effectively ensured the sterility of the final product. The air cleanliness of serum production is 100. All subpackage benches are filtered by high efficiency particulate air filters, and maintain a positive pressure subpackage environment to monitor the total number of particles, suspended particles and pressure. After subpackage, the final product was frozen at - 20 ℃ and isolated until all quality control tests were completed.
4. FBS quality control
Each batch of fetal bovine serum was confirmed to meet the standards, and recorded in the quality inspection report in written form.
5. Quality assurance
The production process of fetal bovine serum was carried out under a strictly controlled condition. Ultraviolet sterilization, filtration, sterilization and sub packaging are the key production links, which can ensure the quality of serum. The data related to serum production is recorded as a special file, from the initial collection of raw materials to the storage of finished products, the whole production process, as well as the quality control test and results are recorded, and the source documents can be traced under monitoring conditions. The production process of fetal bovine serum follows the European Parliament standard for in vitro diagnosis.
Precautions for sv30160.03 fetal bovine serum:
Key points of serum preservation
(1) The serum that needs to be stored for a long time must be stored in the refrigerator at - 20 ℃ - 70 ℃. The storage time in the refrigerator at 4 ℃ should not exceed 1 month. Because the volume of serum will increase by about 10% when it freezes, a certain volume space must be reserved before it freezes into the refrigerator, otherwise it is easy to cause contamination or glass bottle cracking(2) Heat inactivation refers to the complete thawed serum heated at 56 ℃ for 30 minutes. During the heating process, it must be shaken regularly and evenly. The purpose of this heat treatment is to inactivate the complement components in the serum. Unless necessary, this heat treatment is generally not recommended, because heat treatment will cause a significant increase in serum sediment and affect the quality of the serum, Smooth muscle cells contract, mast cells and platelets release histamine, enhance phagocytosis, promote chemotaxis and activation of lymphocytes and macrophages
(3) The serum in the refrigerator from - 20 ℃ to - 70 ℃ should be dissolved in the refrigerator at 4 ℃ for 1 day. Then, it should be moved to room temperature and then sub packed. During the dissolution process, it should be shaken gently and evenly (be careful not to cause bubbles) to make the temperature and composition uniform and reduce the occurrence of precipitation. Do not thaw the serum directly from - 20 ℃ to 37 ℃, Because the temperature changes too much, it is easy to cause protein agglutination and precipitation
(4) Generally, the serum provided by the manufacturer is sterile and does not need to be filtered. If the serum is found to have suspended substance, the serum can be added into the culture medium and filtered together. Do not filter the serum directly
(5) Precipitate floccules in serum: mainly caused by lipoprotein denaturation in serum and fibrin in serum after thawing. These floccules will not affect the quality of serum itself. They can be removed by centrifugation at 3000rpm for 5 minutes or without treatment, In general, the small black spots will not affect cell growth, but if the quality of serum is suspected, it should be stopped immediately and replaced with another batch of serum
(6) Do not store the serum at 37 ℃ for too long, otherwise the serum will become turbid, and the effective components in the serum will be broken, which will affect the quality of serum storage / transportation: - 5 ° C to - 20 ° C.
Fetal bovine serum (North American blood) 100ml / bottle
Fetal bovine serum (Australian blood) 100ml / bottle
Special grade fetal bovine serum 200ml / bottle
Premium fetal bovine serum 200ml / bottle
Standard fetal bovine serum 200ml / bottle
Standard fetal bovine serum 100ml / bottle
Special grade horse serum 200ml / bottle
Standard horse serum 200ml / bottle
Human serum (mixed blood type) 100ml / bottle
Human AB serum (AB blood group) 100ml / bottle
Rabbit serum 200ml / bottle
Goat serum 100ml / bottle
Sheep serum 100ml / bottle
Fetal bovine serum (Australian blood) 100ml / bottle
Special grade fetal bovine serum 200ml / bottle
Premium fetal bovine serum 200ml / bottle
Standard fetal bovine serum 200ml / bottle
Standard fetal bovine serum 100ml / bottle
Special grade horse serum 200ml / bottle
Standard horse serum 200ml / bottle
Human serum (mixed blood type) 100ml / bottle
Human AB serum (AB blood group) 100ml / bottle
Rabbit serum 200ml / bottle
Goat serum 100ml / bottle
Sheep serum 100ml / bottle
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