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Technical Column: Frequently Asked Questions and Answers for Flow Cytometry (FCM)

FCM is a technique that uses flow cytometry to perform individual, multi parameter, and rapid qualitative and quantitative analysis or analysis of single row cells or biological particles in a fast linear flow state. However, in the practical application of FCM, obtaining high-quality streaming data is not an easy task. We will now analyze the common problems in FCM experiments, hoping to help relevant experimenters improve the success rate of the experiments.

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Key points to note in ELISA experiments!

Shanghai Hengyuan Biotechnology Co., Ltd. was established in December 2010, integrating research and development, production, and sales. Its core is the production of scientific research reagents and the supply and production of diagnostic reagent raw materials. It is committed to providing high-quality biological products to customers from universities, research institutions, diagnostic reagent manufacturers, and biopharmaceutical companies worldwide. Hengyuan Biotechnology currently has over 500000 genetic products of various species; More than 200000 types of recombinant proteins; There are over 30000 types of polyclonal antibodies from dozens of species and hundreds of high-quality monoclonal antibodies. Hengyuan brand has independently developed ELISA kits, which now cover over 20 species and thousands of products, involving more than ten research fields such as tumors, hormones, autoimmune, cardiovascular, and metabolism. Hengyuan Biotechnology has summarized that the crucial steps in ELISA kit experiments are:

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Notes on sample processing in ELISA experiments

There are various types of samples detected by ELISA kits, such as serum, plasma, urine, cell culture supernatant, or homogenate, etc. The pre-treatment methods for different types of test samples are different. The processing of experimental samples can be considered one of the key processes in ELISA testing, so research friends must pay more attention. Below we will give you a detailed explanation!

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What do you know about the three signaling pathways of IL-6?

In 1986, IL-6 was identified as a b-cell stimulator that promotes the differentiation of effector B cells into antibody-producing cells. IL-6, also known as IFNΒ2, hepatocyte-stimulating factor and hybridoma/plasmacytoma growth factor, is a 25-kDa-secreting glycopeptide composed of 184 amino acids and monocytes and macrophages are major producers of IL-6. T cells, B cells, liver cells, endothelial cells, fibroblasts, Keratinocyte, mesangial cells and adipocytes, as well as several types of tumor cells can also be formed or stimulated. IL-6 is a pleiotropic cytokine that can affect a variety of immune and physiological processes, such as acute phase protein production, inflammation, antigen Hapten response, hematopoiesis, apoptosis, differentiation and cell metabolism.

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Technical Column: Precautions for Phosphorylated Protein WB Experiment

Protein phosphorylation is a very important post-translational processing, and detecting protein phosphorylation is necessary for signaling pathways. Western blot is the most commonly used method to evaluate protein phosphorylation status, which is not much different from running normal proteins, but incubating antibodies that recognize phosphorylation. Below, Hengyuan Biotech will summarize the key points to pay attention to in the experiment.

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