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Research Information: Differences between Primary Cells, Cell Lines, and Cell Lines

Primary cells refer to cells obtained from tissues of the body through proteases or other methods and cultured in vitro, known as primary cells. It is generally believed that the first generation of cultured cells and cells passaged up to the 10th generation are collectively referred to as primary cell culture.

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Do you know the advantages and disadvantages of blood free culture media?

Each product has its own strengths, and a synthetic culture medium that can reproduce in vitro for a long time without adding any serum is called serum-free culture medium. It has now been widely used to cultivate mammalian cells to prepare monoclonal antibodies, recombinant proteins, and viral antigens. So, what are the advantages and disadvantages of serum-free culture media? Let's take a look with the editor from Hengyuan!

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What should I do if there are bubbles in the enzyme-linked immunosorbent assay kit?

Enzyme linked immunosorbent assay (ELISA), also known as enzyme-linked immunosorbent assay (ELISA), is a widely used method for detecting various antigens and antibodies, with high sensitivity and strong specificity. So, how should the bubbles in the reagent kit be handled correctly in enzyme-linked immunosorbent assay? Let's take a look together!

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WB experiment: What's wrong with a blank on the membrane?

Hengyuan Biotechnology mainly engages in scientific research reagent products and currently has over 500000 types of gene products for various species; More than 200000 types of recombinant proteins; There are over 30000 types of polyclonal antibodies from dozens of species and hundreds of high-quality monoclonal antibodies. Hengyuan brand has independently developed ELISA kits, which now cover over 20 species and thousands of products, involving more than ten research fields such as tumors, hormones, autoimmune, cardiovascular, and metabolism. This week, the editor brings you the results of the WB experiment - a blank on the membrane. What is the reason for this? Let's take a look together!

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Explanation of references cited in the earthworm 8-hydroxydeoxyguanosine (8-OHdG) ELISA kit

[Hengyuan Literature] Reference Explanation: Today, the editor will share with you a relevant literature on the earthworm 8-hydroxydeoxyguanosine (8-OHdG) ELISA kit. This SCI literature was successfully published in the authoritative issue of the Journal of Hazardous Materials - Fosthiazate, a soil applied nematide, induced oxidative stress, Neurotoxicity and transcriptome errors in earthworm (Eisenia fetida), IF impact factor 13.6 points!

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