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Microbial Reactive Oxygen Species (ROS) ELISA Kit Product Description
Detection range:
80 pg/mL -2600 pg/mL
Purpose of use:
This kit is used to determine the content of reactive oxygen species (ROS) in microbial samples.
Experimental principle:
This kit uses the double antibody sandwich method to determine the level of reactive oxygen species (ROS) in specimens. Coat a microplate with purified reactive oxygen species (ROS) antibody to prepare a solid-phase antibody. Add ROS to the micropores coated with the monoclonal antibody in sequence, and then bind with HRP labeled ROS antibody to form an antibody antigen enzyme labeled antibody complex. After thorough washing, add substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. The depth of color is positively correlated with the reactive oxygen species (ROS) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of reactive oxygen species (ROS) in the sample using a standard curve.
Storage conditions and expiration date
1. Storage of reagent kit:; 2-8 ℃.
2. Validity period: 6 months
Advantages of Shanghai Hengyuan Biological Reagent Kit:
1. High cost-effectiveness: High quality, low price.
2. Efficient and flexible: Multiple samples can be analyzed simultaneously, allowing for flexible determination of the number of samples to be analyzed.
3. Strong specificity: Select highly specific paired antibodies.
4. Simple operation: 96 well pre coated plate with complete reagents, short experimental time.
Hengyuan Biotechnology specializes in fetal bovine serum, biological reagents, ELISA kits, culture media, control samples, standard samples, antibodies, and more. We provide high-quality serum from various brands such as Gibco, Hyclone, Nqbb, Sigma, with guaranteed quality and affordable prices. We welcome customers to inquire!
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