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In situ terminal apoptosis (Tunel)

In situ terminal apoptosis (Tunel)

Experimental principle

TUNEL technology can stain apoptotic bodies or single apoptotic cells in tissues or cells, and can accurately reflect the most typical biochemical and morphological characteristics of cell apoptosis. Endogenous endonucleases in apoptotic cells are activated to cut their own chromosomal DNA into gaps or 3-OH end fragments. The use of deoxynucleotide terminal transferase will be marked with markers (such as digoxin, The deoxynucleotide (biotin or fluorescein) is transferred to the 3-OH end, and then the apoptotic cells are detected by immunohistochemistry or immunofluorescence.

Experiment process

Take the paraffin embedded sample as an example:

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