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Real-time fluorescence quantitative PCR

Real-time fluorescence quantitative PCR

Experimental principle

Real-time fluorescent quantitative PCR technology is to add fluorescent dyes or fluorescent probes to the PCR reaction system, use the accumulation of fluorescent signals to monitor the entire PCR reaction process in real time, and finally calculate the target gene expression difference analysis through the internal reference control or quantitatively analyze the unknown template through the standard curve It has the characteristics of simple operation, fast and efficient, high throughput, high sensitivity and so on.

Experiment process

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