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Computer Biochemistry

Computer Biochemistry

   First. Collection and preservation of samples

  1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or 4°C overnight, then centrifuge at 1,000×g for 20 minutes, take the supernatant, and place the supernatant at -20°C or -80 Store at ℃, avoid repeated freezing and thawing.

  2. Plasma: Use EDTA or heparin as an anticoagulant to collect specimens, and centrifuge the specimens at 2-8°C 1,000×g for 15 minutes within 30 minutes after collection. Take the supernatant to test, or place the supernatant in Store at -20℃ or -80℃, avoid repeated freezing and thawing.

  Second. Components and storage conditions of the kit

  1. Main components: R1, R2 (see the corresponding index manual for specific components)

  2. Storage conditions and expiry date: the original packaged kit is stored at 2-8°C, valid for 12 months

  Three, reagent preparation

  In the single-reagent method, R1 and R2 are prepared and mixed according to the manual ratio; in the double-reagent method, R1 and R2 are applied separately.

  Four, operation steps

  1. Preparation before starting

  A. Check the power strip and confirm that the power input switch is turned on.

  B. Check whether the waste liquid bottle is full, if full, please empty it.

  C. Check if the printing paper is enough, if not enough, add printing paper.

  D. Check if the cleaning solution and distilled water are enough, if not enough, please add it.

  E. Confirm that the diluent has been placed in position 39 of the reagent tray and deionized water has been placed in position 40. If it is not placed, please add it.

  F. Confirm that deionized water has been placed on the sample tray at position 40, if not, please add it.

  G. Check the sample needle to confirm that there is no contamination or bend. If there is contamination, scrub the sample needle. If it is bent, replace the sample needle.

  2. Turn on

  After the system is powered on, turn on the power in the following order, the biochemical host power supply, the display power supply, and the computer host power supply.

  3. Start the control software

  After logging in to the Windows operating system, run the Chemray240 automatic biochemical analyzer operating software on the desktop.

  4. Setting parameters

  Before applying for the test, at least the following parameters must be set

  A. Click "Set Parameters", "Project Settings", enter the parameters of the project according to the actual manual, and click "Reference Range" to enter the reference range.

  B. Click "Parameter Settings", "Standard Settings", and enter the parameters of the items to be calibrated according to the actual instructions.

  C. Click "Parameter Setting", "Quality Control Setting", and set the target value and SD of each item according to the quality control setting manual.

  D. Click "Reagent Settings" to set reagent information and reagent position.

  E. Click "System Settings", "System Control Parameters" to set the system temperature and test mode.

  5. Place reagents

  Place the corresponding reagent in the reagent position set on the reagent tray and open the reagent bottle cap. When using the reagent bottle, please select a reagent bottle with the same capacity as the reagent position setting to ensure correct residual detection.

  6. Calibration

  Before use, perform a calibration test. Click "sample application", "standard application" to apply for calibration test items. After applying for calibration, place the calibration solution of the item in the set position on the sample tray. Click "Start" to perform the calibration test. Click "Result Query", "Quality Control Query" to view the calibration results.

  7. Quality control

  Click "sample application", "quality control application" to apply for quality control test items. After applying for quality control, place the quality control solution for the item in the set position on the sample tray. Click "Start" for quality control test. Click "Result Query", "Quality Control Query" to view the quality control results.

  8. Sample analysis

  A. Manual sample test (can be used in any case): Click "Apply for sample" to apply for sample test, and place the corresponding sample on the set position on the sample tray.

  B. Apply for sample testing in batches: Click "Apply for Samples", after editing the test items, click "Save in Batches", enter the start sample bit and end sample bit, and click "OK".

  After applying for the sample, place the corresponding sample in the set position on the sample tray. Click "Start" to perform sample test. Click "Test Status" and "Test List" to view the real-time results of the sample. Click "Result Query" and "Patient Record" to view the sample test results.

  9. Transfer sample results

  Click "Result Query" and "Patient Record" to select the sample record to be transmitted, and then click the "Transfer" button.

  10. Exit the operating software and shut down

  After all the tests are completed, when the system is in the standby state, you can click Shut Down, select Exit, OK, and exit the operating software. After exiting the Windows operating system, turn off the power in the order of the monitor power supply and the biochemical host power supply.

  11. Operation after shutdown

  A. Close the lid of each reagent bottle in the reagent tray.

  B. Take away the calibration solution, quality control solution and sample from the sample tray.

  C. Check whether there is any contamination on the analyzer table, if so, wipe off the stain with a clean soft cloth.

  D. Check the waste container, if necessary, empty the waste container.

  Five. Calculation of biochemical results

  The result of the automatic biochemical analysis detection index is that the instrument automatically generates the sample concentration and can export it to excel.

  Six, matters needing attention

  1. Sample requirements: The sample should be clear and transparent, and the suspended matter should be removed by centrifugation; avoid using hemolytic samples. If the samples are not tested immediately after collection, they should be frozen and stored in a -80° refrigerator to avoid repeated freezing and thawing. There should be no air bubbles during the sample detection, so as to prevent the sampling needle from sucking the air bubbles and thinking that the sample is inhaled. Sample hemolysis has an impact on the experiment, and timely feedback is required. The sample size should be sufficient, and the bottom volume (about 100ul) should be reserved for testing.

  2. Operational requirements: the reagent preparation must be accurate, the sample amount must be accurate, and the sample and reagent in the pipette must be completely drained; the configured reagent should be poured into the reagent bottle to avoid bubbles, so as to prevent the sampling needle from inhaling bubbles and thinking of inhalation Reagents. The order of the indicators on the computer will affect the results of the experiment, that is, the composition of the reagents in the kit or the color reaction will affect the detection results of other indicators, just like the magnesium-containing reagent in alkaline phosphatase will affect the detection of magnesium ions; partly The detection items containing potassium in the reagent will cause errors in the enzymatic detection of potassium; the detection of creatine kinase and creatine kinase isoenzymes will cause false increases in the determination of glucose, etc. Therefore, it is necessary to arrange the computer sequence before the experiment to avoid mutual interference between experiments.

  3. Safety requirements: Do not touch the sample reagent tray during testing to avoid being injured by the sampling needle.

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