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What is the operating principle of PCR technology?
The basic principle is similar to the natural replication process of DNA, and its specificity depends on oligonucleotide primers complementary to both ends of the target sequence.
PCR consists of three basic reaction steps: denaturation, annealing, and elongation
① Denaturation of template DNA: After heating the template DNA to around 94 ℃ for a certain period of time, the double stranded template DNA or the double stranded DNA formed by PCR amplification dissociates into single strands, allowing it to bind with primers and prepare for the next round of reaction;
② Annealing (refolding) of template DNA and primers: After heating and denaturation of template DNA into single strands, the temperature drops to around 55 ℃, and the complementary sequence of primers and template DNA single strands pairs and binds;
③ Primer extension: Under the action of Taq enzyme, DNA template primer conjugates use dNTP as the reaction material, target sequence as the template, and synthesize a new semi preserved replication chain complementary to the template DNA chain according to the principles of base pairing and semi preserved replication.
By repeating the three processes of denaturation annealing extension, more "semi preserved replicated chains" can be obtained, and this new chain can also serve as a template for the next cycle. It takes 2-4 minutes to complete each cycle, and within 2-3 hours, the target gene can be amplified by several million times.
For more experimental information, please consult our sales representative. Our company provides comprehensive experimental technology services, greatly saving the time and energy of repeated labor for scientific researchers. We have excellent technical consultation and perfect after-sales service. Purchasing ELISA reagent kits comes with free testing services and online one-on-one technical guidance to solve your worries.
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