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Northern hybridization
Experimental principle
Northern blot hybridization is a DNA/RNA hybridization. It is a technique for detecting specific RNA. The RNA mixture is first separated by denaturing agarose gel electrophoresis according to their size and relative molecular weight. The RNA after gel separation Transfer to a nylon membrane or nitrocellulose membrane by southern blotting, and then hybridize with the labeled probe. The transcription expression can be quantitatively or qualitatively analyzed through hybridization result analysis.
Experiment process
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- Immunological testing
- Biochemical testing
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- Cell experiment
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