Welcome to choose us, Shanghai Hengyuan Biological Technology Co., Ltd.!Free service hotline:400-0899-208
Language:CH Security check
Methylation
Experimental principle
DNA methylation exists in most eukaryotes, and generally occurs in the 5'UTR region of cytosine in CpG dinucleotides, which plays a regulatory role. About 80% of the cytosine of CpG is methylated, and 20% is in the CpG island of the gene regulatory region. The principle of BSP methylation sequencing (Bisulfite Genomic Sequencing PCR) is through bisulfite treatment of genomic DNA. Unmethylated cytosine is deaminated and converted into uracil. In the subsequent PCR reaction, uracil is converted into Thymine, and methylated cytosine cannot be deaminated and is retained when the reaction is completed. We will perform TA cloning and sequencing on the amplified PCR products to analyze the specific conditions of methylation. This method has high accuracy and is the gold standard for methylation detection.
Services
- Immunological testing
- Biochemical testing
- Pathology testing
- Molecular biology testing
- Cell experiment
Latest news
- What are the methods for culturing vascular smooth muscle cells with fetal bovine serum?
- What is the operating principle of PCR technology?
- Congratulations to the teachers of Hebei Agricultural University for receiving the scholarship!
- What is the difference between standard and control samples?
- New Product Recommendation: Hengyuan Bio human ELISA Kit Recommendation Guide
- Key points of ELISA kit for interleukin experiments
- How to prevent the formation of black spots in fetal bovine serum culture
- Is the sensitivity of ELISA kit low?
- Leading the opening season, Hengyuan Happy Shopping