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Hengyuan Biotechnology | Key points of ELISA experiment - incubation
The incubation in ELISA (enzyme-linked immunosorbent assay) experiments is a crucial step that directly affects the binding efficiency of antigens and antibodies on the solid surface, thereby affecting the accuracy and reliability of the entire experiment. Here are the detailed points about incubation in ELISA experiments. Let's take a look together!
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What are the methods for culturing vascular smooth muscle cells with fetal bovine serum?
The commonly used methods for culturing vascular smooth muscle cells with fetal bovine serum include the probe method and the enzyme dispersion method. Let's take a look at these two methods together.
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What is the operating principle of PCR technology?
The basic principle is similar to the natural replication process of DNA, and its specificity depends on oligonucleotide primers complementary to both ends of the target sequence.
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What is the difference between standard and control samples?
A standard substance, also known as a standard item, serves as a measurement standard. If used in the field of medicine, it is the standard content in content determination. Standards include chemical metrology standards, metallurgical standards, and drug testing standards. Using biochemical methods for determination, there are a total of 15 national standards, including lincomycin, neomycin, spectinomycin, tylosin, streptomycin, kanamycin, chorionic gonadotropin, salinase, bacitracin zinc, guitarin, ampicillin, erythromycin, synthetic oxytocin, gentamicin, and naringin G.
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Key points of ELISA kit for interleukin experiments
There are various types of samples for detecting interleukins in ELISA kits, such as serum, plasma, urine, cell culture supernatant, or tissue homogenate. The preparation methods for different types of test samples are different. The processing of experimental samples can be regarded as one of the key processes in ELISA experiments, so scientific research friends must pay more attention.
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- What are the methods for culturing vascular smooth muscle cells with fetal bovine serum?