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Notes on sample processing in ELISA experiments
There are various types of samples detected by ELISA kits, such as serum, plasma, urine, cell culture supernatant, or homogenate, etc. The pre-treatment methods for different types of test samples are different. The processing of experimental samples can be considered one of the key processes in ELISA testing, so research friends must pay more attention. Below we will give you a detailed explanation!: View details

What do you know about the three signaling pathways of IL-6?
In 1986, IL-6 was identified as a b-cell stimulator that promotes the differentiation of effector B cells into antibody-producing cells. IL-6, also known as IFNΒ2, hepatocyte-stimulating factor and hybridoma/plasmacytoma growth factor, is a 25-kDa-secreting glycopeptide composed of 184 amino acids and monocytes and macrophages are major producers of IL-6. T cells, B cells, liver cells, endothelial cells, fibroblasts, Keratinocyte, mesangial cells and adipocytes, as well as several types of tumor cells can also be formed or stimulated. IL-6 is a pleiotropic cytokine that can affect a variety of immune and physiological processes, such as acute phase protein production, inflammation, antigen Hapten response, hematopoiesis, apoptosis, differentiation and cell metabolism.: View details

Technical Column: Precautions for Phosphorylated Protein WB Experiment
Protein phosphorylation is a very important post-translational processing, and detecting protein phosphorylation is necessary for signaling pathways. Western blot is the most commonly used method to evaluate protein phosphorylation status, which is not much different from running normal proteins, but incubating antibodies that recognize phosphorylation. Below, Hengyuan Biotech will summarize the key points to pay attention to in the experiment.: View details

Research Information: Differences between Primary Cells, Cell Lines, and Cell Lines
Primary cells refer to cells obtained from tissues of the body through proteases or other methods and cultured in vitro, known as primary cells. It is generally believed that the first generation of cultured cells and cells passaged up to the 10th generation are collectively referred to as primary cell culture.: View details

Do you know the advantages and disadvantages of blood free culture media?
Each product has its own strengths, and a synthetic culture medium that can reproduce in vitro for a long time without adding any serum is called serum-free culture medium. It has now been widely used to cultivate mammalian cells to prepare monoclonal antibodies, recombinant proteins, and viral antigens. So, what are the advantages and disadvantages of serum-free culture media? Let's take a look with the editor from Hengyuan!: View details

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