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Technical dry goods: medium sterilization depends on the elements


　　The culture medium is made of different materials, the application requirements are different, and the storage and storage are also slightly different. Generally, the culture medium is easily contaminated or differentiated by bacteria after being heated and absorbed by moisture. Therefore, the culture medium must be kept in a cool place, protected from moisture, light, and humidity. For some culture media that require severe sterilization (such as arranging culture media), they must be stored in a refrigerator at 3-6°C for a longer period of time. Because the liquid is not easy to keep for a long time, it is changed into powder. It is an artificially made nutrient for the growth and maintenance of microorganisms, plants and animals. Whether sterilization is complete or not has many influencing factors. In addition to the variety and quantity of bacteria in itself, the unevenness of sterilization temperature, and the length of time, it is also It depends on the following factors.

　　Whether the medium can be sterilized completely depends on the following factors:

　　1. Is the air in the sterilization pot drained?

　　This is the key to the proportional relationship between temperature and pressure that affects sterilization. When the same pressure is reached, if the air is not purged, that is to say, it is not pure steam sterilization, the temperature at the moment can reach the intended request. , Will seriously affect the sterilization effect.

　　2, moist heat sterilization

　　When 　　 moist heat sterilization, the microorganisms are killed, and the nutrients are also damaged, especially amino acids and vitamins. For example, at 121°C for only 20 minutes, 59% of lysine, arginine and other basic amino acids were damaged, and an appropriate amount of methionine and tryptophan were also damaged. Under the effect of heat, certain nutrients may also react to each other due to heat, which constitutes a change in the quantity of the original nutrients in the medium, thus affecting its quality.

　　3, foam

　　The air in the foam forms a thermal insulation layer, which makes it difficult for heat to penetrate and kill the bacteria in it.

　　4, particles

　　The particles are small and easy to sterilize, while the large particles are difficult to sterilize. For those containing a few larger particles and coarse fibers, coarse filtration can be used to remove them.

　　5, pH value

　　The pH value has a great influence on the heat resistance of microorganisms. When the pH is between 6.0 and 8.0, microorganisms are least likely to die. When the pH is less than 6.0, the microorganisms die relatively easily. At this moment, H+ easily enters the cells of the microorganisms, thereby changing the physiological response of the cells and prompting their death. Therefore, the lower the pH of the medium, the shorter the time required.

　　Shanghai Hengyuan Hengyuan specializes in domestic/imported elisa kits, domestic/imported serums, standard/reference substances, biochemical reagents, culture media, antibodies, cells, cell culture and other scientific research products. Provide free product quotations, experimental principles, product usage and instructions in both Chinese and English. For many years, our company has provided comprehensive and thoughtful procurement services for various universities, various hospitals, bioengineering medical laboratories, and other industry research consumers. If you need to experiment, please come to consult and buy!


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