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Monkey ELISA kit
- Monkey interleukin 12 (IL-12 / P40) ELISA Kit
- Shanghai Hengyuan biology has been engaged in ELISA for more than 10 years with mature technology and stable products. Hengyuan brand has independently developed ELISA kits, covering more than 20 species and thousands of products, involving more than 10 research fields such as tumor, hormone, autoimmune, cardiovascular, metabolism, etc. Welcome to inquire.
|
Chinese name |
Monkey interleukin 12 (IL-12 / P40) ELISA Kit |
|
Specifications |
96T |
|
Article number |
HB078-Mk |
|
Price |
￥1850 |
|
Species and genus |
monkey |
|
Sample type |
Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant, etc |
|
Detection range |
See the instruction manual for details |
|
sensitivity |
See the instruction manual for details |
|
reaction time |
1-5h |
|
Sample volume |
10ul |
|
Detection wavelength |
450nm |
|
Delivery date |
3-5day |
|
instructions |
Consult our salesman for information |
[precision]
The precision was expressed by CV of the measured value. CV（%） = SD/mean×100
Intra batch difference: take the same batch of kits for quantitative detection of low, medium and high fixed value samples, each sample was determined for 20 times continuously, and calculate the average value and SD value of samples with different concentrations.
Inter batch difference: three different batches of kits were selected for quantitative determination of low, medium and high value samples, and each sample was determined 10 times with the same kit, and the average value and SD value of samples with different concentrations were calculated.
Intra assay difference: CV < 6.3%
Inter batch difference: CV < 9.4%
[recovery rate]
Five different samples were added with known concentration of target protein, and recovery experiments were carried out to get the recovery range and average recovery.
|
Sample Type |
Range (%) |
Average Recovery (%) |
|
Tissue Lysates (n=5) |
92-102 |
98 |
|
Serum (n=5) |
91-106 |
98 |
|
EDTA plasma (n=5) |
90-108 |
102 |
|
Cell culture media (n=5) |
87-107 |
101 |
[linear]
Five samples were added with known concentration of target protein, and recovery experiments were carried out to get the recovery range and average recovery. 5 samples were diluted 2 times, 4 times, 8 times, 16 times to do the recovery experiment, the recovery range and average recovery were obtained.
|
Sample |
1:2 |
1：4 |
1:8 |
1:16 |
|
Tissue Lysates (n=5) |
92-105% |
84-95% |
90-102% |
80-93% |
|
Serum (n=5) |
81-103% |
89-98% |
86-97% |
83-101% |
|
EDTA plasma (n=5) |
85-98% |
82-104% |
83-96% |
79-91% |
|
Cell culture media (n=5) |
82-105% |
89-113% |
81-106% |
82-110% |
[specificity]
The kit identified the natural and recombinant monkey interleukin 12 (il-12/p40), and there was no obvious cross reaction with other similar substances. Due to the limitation of technology and sample source, it is impossible to complete cross reaction detection of all related or similar substances, so it is possible that the reagent box has cross reaction with other substances not tested.
[stability]
After the determination, the kit must be kept at the recommended temperature within the validity period, and the activity reduction rate is less than 5%. In order to reduce the influence of external factors on the detection value before and after the destruction of the kit, the environmental conditions of the laboratory should be kept as consistent as possible, especially the temperature, humidity and temperature education conditions in the laboratory. Secondly, the same experimenter can reduce the human error.
[kit composition]
|
Serial number |
Chinese name |
English name |
Specifications |
|
1 |
30 times concentrated detergent |
Wash solution(30X) |
20ml×1瓶 |
|
2 |
Enzyme labeling reagent |
HRP-Conjugate reagent |
6ml×1瓶 |
|
3 |
Enzyme coated plate |
Microelisa stripplate |
12孔×8条 |
|
4 |
Sample diluent |
Sample diluent |
6ml×1瓶 |
|
5 |
Developer a solution |
Chromogen Solution A |
6ml×1瓶 |
|
6 |
Developer B solution |
Chromogen Solution B |
6ml×1/瓶 |
|
7 |
Termination solution |
Stop solution |
6ml×1瓶 |
|
8 |
Standard |
Standard |
0.5ml×1瓶 |
|
9 |
Standard diluent |
Standard diluent |
1.5ml×1瓶 |
|
10 |
instructions |
Product Description |
1份 |
|
11 |
Sealing film |
Sealing plate membrane |
2张 |
|
12 |
Sealed bag |
Sealing bag |
1个 |
Note: please check whether the label and quantity of reagents in the kit are consistent with the table before use.
Materials and consumables required but not provided
1. Microplate reader (it is recommended to preheat the reader before use)
2. Precision pipette, suction head and sampling tank
3. Distilled or deionized water
4. Bottle washing or automatic plate washing machine
5. 37 ℃ water bath or Incubator
6. EP tube
7. Powder free disposable latex gloves
[storage conditions and validity period]
1. 2-8 ℃, do not freeze, valid for 6 months.
2. After use, the coated microplate is put into the self sealing bag with desiccant, the self sealing bag is sealed, and all reagents are put back to the refrigerator at 2-8 ℃.
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