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Rat ELISA kit
- Rat glycogen synthase kinase-3 β( GSK-3 β） ELISA Kit
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Shanghai Hengyuan Biology has been engaged in enzyme-linked immunology for more than 10 years, with mature technology and stable products. Hengyuan brand independently develops ELISA kits, which now cover more than 20 species and thousands of products, involving more than ten research fields such as tumors, hormones, autoimmunity, cardiovascular, and metabolism. Its ELISA kit is not only rich in detection indicators, but also can customize the detection range according to experimental needs, and provide experimental outsourcing and free testing services. You are welcome to inquire.
|
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Rat glycogen synthase kinase-3 β( GSK-3 β） ELISA Kit |
| Specifications | 96T |
| article number |
HB306-Ra |
| Price | ￥1800 |
| Species | rat |
| Sample type | serum, plasma, tissue homogenate,cell lysate, cell Culture supernatant etc. |
| Detection range | See the manual for details |
| Sensitivity | See the manual for details |
| Response time | 1-5h |
| Sample volume | 10ul |
| Detection wavelength | 450nm |
| Delivery date | 3-5 days |
| Manual | Consult our salesperson for obtaining |
The precision is expressed by the coefficient of variation CV of the measured value of the sample. CV (%) = SD/mean×100
Intra-batch difference: Take the same batch of kits for quantitative detection of low, medium, and high value fixed value samples. Each sample is measured 20 times continuously, and the average value and SD value of samples of different concentrations are calculated.
Inter-batch difference: select 3 different batches of kits to quantitatively determine the low, medium, and high-value fixed value samples. Use the same kit for each sample to repeat the measurement 10 times, and calculate the average value and SD of samples with different concentrations. value.
Intra-run difference: CV<4.5%
Difference between batches: CV<7.4%
【Recovery rate】
The target protein of known concentration was added to 5 different samples, and the recovery experiment was performed to obtain the recovery range and average recovery rate.
|
Sample Type |
Range (%) |
Average Recovery (%) |
|
Tissue Lysates (n=5) |
93-107 |
100 |
|
Serum (n=5) |
95-106 |
102 |
|
EDTA plasma (n=5) |
94-105 |
94 |
|
Cell culture media (n=5) |
82-108 |
101 |
【Linear】
Add the target protein of known concentration to 5 samples respectively, do recovery experiment, get the range of recovery rate and average recovery rate. Dilute 5 samples by 2 times, 4 times, 8 times, and 16 times respectively for recovery experiments to obtain the recovery range and average recovery rate.
|
Sample |
1:2 |
1：4 |
1:8 |
1:16 |
|
Tissue Lysates (n=5) |
92-105% |
84-99% |
90-102% |
80-93% |
|
Serum (n=5) |
81-101% |
89-99% |
86-98% |
83-101% |
|
EDTA plasma (n=5) |
85-97% |
82-101% |
83-96% |
79-91% |
|
Cell culture media (n=5) |
83-105% |
89-112% |
81-106% |
82-110% |
【Specificity】
This kit recognizes natural and recombinant Rat glycogen synthase kinase-3 β( GSK-3 β）, and has no obvious cross-reactivity with other similar substances. Due to the limitation of technology and sample source, it is impossible to complete the cross-reaction test of all related or similar substances. Therefore, this kit may have cross-reaction with other untested substances.
【Stability】
It has been determined that the kit must be stored at the recommended temperature within the validity period, and the activity reduction rate is less than 5%. In order to reduce the influence of external factors on the detection value before and after the destruction of the kit, the environmental conditions of the laboratory should be kept as consistent as possible, especially the temperature, humidity and incubation conditions in the laboratory. Secondly, the operation by the same experimenter can reduce human error.
【Kit composition】
|
Serial number |
English name |
specification |
|
1 |
Wash solution(30X) |
20ml×1 bottle |
|
2 |
HRP-Conjugate reagent |
6ml×1 bottle |
|
3 |
Microelisa stripplate |
12 holes×8 strips |
|
4 |
Sample diluent |
6ml×1 bottle |
|
5 |
Chromogen Solution A |
6ml×1 bottle |
|
6 |
Chromogen Solution B |
6ml×1/bottle |
|
7 |
Stop solution |
6ml×1 bottle |
|
8 |
Standard |
0.5ml×1 bottle |
|
9 |
Standard diluent |
1.5ml×1 bottle |
|
10 |
Product Description |
1 serving |
|
11 |
Sealing plate membrane |
2 sheets |
|
12 |
Sealing bag |
1 piece |
Note: Please check whether the label and quantity of the reagents in the kit are consistent with the table before use.
Materials and consumables needed but not provided
1. Microplate reader (it is recommended to warm up the instrument before use)
2. Precise pipettes, tips, and sample tanks
3. Distilled or deionized water
4. Bottle washing or automatic plate washing machine
5. 37℃ water bath or thermostat
6. EP tube
7. Powder-free disposable latex gloves
【Storage conditions and validity period】
1. Store at 2-8℃, do not freeze, the validity period is 6 months.
2. After opening and use, put the coated microplate into a ziplock bag with desiccant, seal the ziplock bag, and put all reagents back into the refrigerator at 2-8°C.
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