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Sheep ELISA kit
- Sheep 1,25 dihydroxyvitamin D3 (1,25 - (OH) 2D3) ELISA Kit
- Shanghai Hengyuan biology has been engaged in ELISA for more than 10 years with mature technology and stable products. Hengyuan brand has independently developed ELISA kits, covering more than 20 species and thousands of products, involving more than 10 research fields such as tumor, hormone, autoimmune, cardiovascular, metabolism, etc. Its ELISA Kit not only has rich detection indicators, but also can customize the detection range according to the experimental needs, and provide experimental outsourcing and free testing services. Welcome to inquire.
name
ELISA kit for sheep 1,25 dihydroxyvitamin D3 (1,25- (OH) 2D3)
Specifications
96T
Article number
HB090-Sp
Price
￥1850
Species and genus
sheep
Sample type
Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant, etc
Detection range
See the instruction manual for details
sensitivity
See the instruction manual for details
reaction time
1-5h
Sample volume
10ul
Detection wavelength
450nm
Delivery date
3-5天
instructions
Consult our salesman for information
[precision]
The precision was expressed by CV of the measured value. CV（%） = SD/mean×100
Intra batch difference: take the same batch of kits for quantitative detection of low, medium and high fixed value samples, each sample was determined for 20 times continuously, and calculate the average value and SD value of samples with different concentrations.
Inter batch difference: three different batches of kits were selected for quantitative determination of low, medium and high value samples, and each sample was determined 10 times with the same kit, and the average value and SD value of samples with different concentrations were calculated.
Intra assay difference: CV < 4.6%
Inter batch difference: CV < 8.8%
[recovery rate]
Five different samples were added with known concentration of target protein, and recovery experiments were carried out to get the recovery range and average recovery.
|
Sample Type |
Range (%) |
Average Recovery (%) |
|
Tissue Lysates (n=5) |
93-103 |
98 |
|
Serum (n=5) |
91-101 |
99 |
|
EDTA plasma (n=5) |
80-109 |
102 |
|
Cell culture media (n=5) |
82-107 |
101 |
[linear]
Five samples were added with known concentration of target protein, and recovery experiments were carried out to get the recovery range and average recovery. 5 samples were diluted 2 times, 4 times, 8 times, 16 times to do the recovery experiment, the recovery range and average recovery were obtained.
|
Sample |
1:2 |
1：4 |
1:8 |
1:16 |
|
Tissue Lysates (n=5) |
92-105% |
84-95% |
90-102% |
80-93% |
|
Serum (n=5) |
81-103% |
89-99% |
86-98% |
83-101% |
|
EDTA plasma (n=5) |
85-97% |
82-101% |
83-96% |
79-91% |
|
Cell culture media (n=5) |
83-105% |
89-112% |
81-106% |
82-110% |
[specificity]
The kit can recognize natural and recombinant sheep 1,25 dihydroxyvitamin D3 (1,25 - (OH) 2D3), and has no obvious cross reaction with other similar substances. Due to the limitation of technology and sample source, it is impossible to complete the cross reaction detection of all related or similar substances. Therefore, the kit may cross react with other substances that have not been detected.
[stability]
The results showed that the kit must be stored at the recommended temperature within the validity period, and the activity reduction rate was less than 5%. In order to reduce the influence of external factors on the test values before and after the destruction of the kit, the environmental conditions of the laboratory should be consistent as far as possible, especially the temperature, humidity and incubation conditions in the laboratory. Secondly, the same experimenter can reduce the human error.
【试剂盒组成】
|
序号 |
中文名称 |
英文名称 |
规格 |
|
1 |
30倍浓缩洗涤液 |
Wash solution(30X) |
20ml×1瓶 |
|
2 |
酶标试剂 |
HRP-Conjugate reagent |
6ml×1瓶 |
|
3 |
酶标包被板 |
Microelisa stripplate |
12孔×8条 |
|
4 |
样品稀释液 |
Sample diluent |
6ml×1瓶 |
|
5 |
显色剂A液 |
Chromogen Solution A |
6ml×1瓶 |
|
6 |
显色剂B液 |
Chromogen Solution B |
6ml×1/瓶 |
|
7 |
终止液 |
Stop solution |
6ml×1瓶 |
|
8 |
标准品 |
Standard |
0.5ml×1瓶 |
|
9 |
标准品稀释液 |
Standard diluent |
1.5ml×1瓶 |
|
10 |
说明书 |
Product Description |
1份 |
|
11 |
封板膜 |
Sealing plate membrane |
2张 |
|
12 |
密封袋 |
Sealing bag |
1个 |
注意：使用前请检查试剂盒中试剂的标签和数量与表格是否一致。
Materials and consumables required but not provided
1. Microplate reader (it is recommended to preheat the reader before use)
2. Precision pipette, suction head and sampling tank
3. Distilled or deionized water
4. Bottle washing or automatic plate washing machine
5. 37 ℃ water bath or Incubator
6. EP tube
7. Powder free disposable latex gloves
[storage conditions and validity period]
1. 2-8 ℃, do not freeze, valid for 6 months.
2. After use, the coated microplate is put into the self sealing bag with desiccant, the self sealing bag is sealed, and all reagents are put back to the refrigerator at 2-8 ℃.
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