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What should I do if there are bubbles in the enzyme-linked immunosorbent assay kit?


Enzyme linked immunosorbent assay (ELISA), also known as enzyme-linked immunosorbent assay (ELISA), is a widely used method for detecting various antigens and antibodies, with high sensitivity and strong specificity. So, how should the bubbles in the reagent kit be handled correctly in enzyme-linked immunosorbent assay? Let's take a look together!

Treatment method for reagent kit bubbles:
1. Use a hair dryer, turn it on and adjust it to the highest temperature. Blow on the 96 well plate for 1-2 seconds.
2. Use better material boards or use specialized 96 hole board oscillators.
3. It can be removed with a tip, but the experiment is laborious and the effect is not yet ideal.
4. Use a syringe needle to puncture.

Precautions for enzyme-linked immunosorbent assay:
1. Whether it is a reagent kit or other products, carefully read the instructions before use and ensure that the reagent kit is within its expiration date.
2. Specimen preparation should be standardized, and each specimen should be prepared (stored) in a volume of 2-3 or more wells. It is best to divide and make backups. For those who cannot be tested in the short term, attention should be paid to low-temperature storage.
3. Determine the required amount of reagent kit based on the number of test specimens.
4. Balance the used reagent kit to room temperature according to the instructions, prepare the required reagent kit, and follow the common technical guidelines for DIY sandwich ELISA.
5. When selecting antibodies, it is best to pair a monoclonal antibody with a multi antibody. If two monoclonal antibodies are selected, they must recognize antibodies with different epitopes, and it is best to choose antibodies from the same company.
Hengyuan Biotechnology has been focusing on the research and sales of research kits for many years, covering various fields of life sciences such as molecular biology, cell biology, immunology, etc., to meet your experimental needs and achieve a win-win situation with customers. With a complete inventory and supply system, as well as efficient and stable patented purification technology, we ensure that our products can be supplied in stock. Looking forward to your call.
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