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Standard curve drawing
Drawing method of standard curve
Various drawing software can be used to draw the ELISA standard curve, the following is an example of Curve Expert1.4 software, the method of drawing the ELISA standard curve is as follows
start up“Curve Expert”
2．Enter the OD value of the standard product on the X axis, and enter the corresponding concentration value on the Y axis, as shown in the figure:
3．Click [Run] 
Button, the following dialog box appears
Button, the following dialog box appears
4．Click the [ok] button, the following two dialog boxes appear, close the following dialog box
5. Some curve names appear in the upper right corner of the dialog box. Click on the curve names from "1", and the corresponding fitted curve will appear in the lower right corner.
6. Select the curve with the best ELISA fit according to the fitted curve and double-click, the following dialog box appears:
Note: Choose the curve equation with the best coefficient (ie, "r" value) for calculation.
"R" value in the upper right corner 
, When the value of "r" is closer to 1, the better the fit
, When the value of "r" is closer to 1, the better the fit
7．Press [Ctrl] key + [L] key, the following dialog box appears:
8．Enter the standard OD value and click [Calculate] to get the actual content of the protein to be tested(The specimen is diluted N times, the calculated value should be multiplied by N).
9. If you want to get the equation of the ELISA fitting curve, right-click in the blank of the dialog box in step 6, and select "Information"
10. Get the following dialog box: click "Copy"
Paste in the place you need to get the following data:
Rational Function: y=(a+bx)/(1+cx+dx^2)
Coefficient Data:
a = -21.531017
b = 212.05019
c = -0.24054479
d = 0.018060778
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