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Selection and optimization of antibody dilution of Abcam
Our company is the designated Abcam brand antibody agent, the price is reasonable, the quality is good! And the use of technical guidance services, Hengyuan provides you with authentic Abcam antibody reagents.
What dilution is used
The binding rate between antibody and antigen depends on the affinity constant. The affinity constant can be affected by temperature, pH and buffer composition. The formation of antibody antigen complexes can also be controlled by changing the relative concentration of antibody and antigen in solution. Since it is usually impossible to change the concentration of the antigen, the optimal working concentration (dilution) of each antibody must be determined for each application and each experimental condition.
Many Abcam antibodies have recommended dilutions for different experiments in the data sheet. We recommend trying to start with these dilutions. However, these dilutions may require some optimization.
Optimization of antibody dilution: titration experiment
The best titer or dilution of Zui is the concentration (dilution) to obtain the best dyeing effect with the least background / nonspecific combination. The best antibody concentration for each assay must be determined by experiment. It is usually determined by using a series of dilutions in titration experiments.
When a new antibody is received in the laboratory, it is recommended to try to determine the best antibody dilution by titration test to obtain the best result.
In order to carry out titration experiment, the first step is to select a fixed incubation time. Then a series of antibodies with experimental dilution were prepared for testing. Each dilution should be tested for the same type of sample to keep the experimental conditions standardized.
Many antibodies have little difference in performance between batches, so you only need to carry out a titration test. However, the concentration of polyclonal antiserum antibody may be significantly different among different animals or at different time of serum collection. In these cases, or when you notice a change in staining results between batches of any antibody, we recommend that the antibody be titrated again to obtain the optimal concentration.
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