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Notes on sample processing in ELISA experiments

There are various types of samples detected by ELISA kits, such as serum, plasma, urine, cell culture supernatant, or homogenate, etc. The pre-treatment methods for different types of test samples are different. The processing of experimental samples can be considered one of the key processes in ELISA testing, so research friends must pay more attention. Below we will give you a detailed explanation!

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What do you know about the three signaling pathways of IL-6?

In 1986, IL-6 was identified as a b-cell stimulator that promotes the differentiation of effector B cells into antibody-producing cells. IL-6, also known as IFNΒ2, hepatocyte-stimulating factor and hybridoma/plasmacytoma growth factor, is a 25-kDa-secreting glycopeptide composed of 184 amino acids and monocytes and macrophages are major producers of IL-6. T cells, B cells, liver cells, endothelial cells, fibroblasts, Keratinocyte, mesangial cells and adipocytes, as well as several types of tumor cells can also be formed or stimulated. IL-6 is a pleiotropic cytokine that can affect a variety of immune and physiological processes, such as acute phase protein production, inflammation, antigen Hapten response, hematopoiesis, apoptosis, differentiation and cell metabolism.

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Chinese Academy of Medical Sciences teachers receive scholarships

I hope the following literature can help you understand the current research progress and our core technologies. Teachers are welcome to contact us for consultation and provide feedback. Through literature, we can also have a better understanding of the product. If you have also published SCI literature using the Hengyuan brand ELISA kit, we welcome you to actively participate in our "Winning Scholarship through Paper Publishing" activity. Perhaps you will be the next recipient of the scholarship! Service hotline: 400-0899-208

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Technical Column: Precautions for Phosphorylated Protein WB Experiment

Protein phosphorylation is a very important post-translational processing, and detecting protein phosphorylation is necessary for signaling pathways. Western blot is the most commonly used method to evaluate protein phosphorylation status, which is not much different from running normal proteins, but incubating antibodies that recognize phosphorylation. Below, Hengyuan Biotech will summarize the key points to pay attention to in the experiment.

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Research Information: Differences between Primary Cells, Cell Lines, and Cell Lines

Primary cells refer to cells obtained from tissues of the body through proteases or other methods and cultured in vitro, known as primary cells. It is generally believed that the first generation of cultured cells and cells passaged up to the 10th generation are collectively referred to as primary cell culture.

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