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Technical Column: Precautions for Phosphorylated Protein WB Experiment
Protein phosphorylation is a very important post-translational processing, and detecting protein phosphorylation is necessary for signaling pathways. Western blot is the most commonly used method to evaluate protein phosphorylation status, which is not much different from running normal proteins, but incubating antibodies that recognize phosphorylation. Below, Hengyuan Biotech will summarize the key points to pay attention to in the experiment.
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Research Information: Differences between Primary Cells, Cell Lines, and Cell Lines
Primary cells refer to cells obtained from tissues of the body through proteases or other methods and cultured in vitro, known as primary cells. It is generally believed that the first generation of cultured cells and cells passaged up to the 10th generation are collectively referred to as primary cell culture.
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The China Pharmaceutical University signed a sales contract with us
Shanghai Hengyuan Biotechnology Co., Ltd. has been focusing on life sciences and biomedical fields for thirteen years, and is a professional biotechnology company that integrates research and development, production, and sales. The company has a comprehensive experimental technology development laboratory, a mature antibody research and development system, proficient in various biological technologies, and combined with the company's R&D team, can effectively ensure that the company's products have strong stability and high accuracy, while also having competitive prices. At present, the company can provide a variety of ELISA detection kits, cells, imported conventional biochemical reagents, antibodies, chemical reagents, pharmacopoeia grade reagents, standards, serum and other products, covering various fields of life sciences such as molecular biology, biochemistry, cell biology, immunology, proteomics, biopharmaceutical and diagnostic reagent research and production.
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Congratulations to Teacher Wang for receiving the scholarship, SCI literature score of 29.4!
Let's congratulate Mr. Wang from the Sixth People's Hospital of Shanghai on successfully passing the SCI literature "Targeted Macrophage CRISPR-Cas13 mRNA Editing in Immunotherapy for Tendon Trial" published in the authoritative journal "ADVANCED MATERIALS" using the independent brand ELISA kit of Hengyuan Biotechnology. The IF impact factor is as high as 29.4 points! Congratulations to Teacher Wang for successfully winning the Hengyuan Biology Scholarship, and thank you for your trust and support in Hengyuan Biology.
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Do you know the advantages and disadvantages of blood free culture media?
Each product has its own strengths, and a synthetic culture medium that can reproduce in vitro for a long time without adding any serum is called serum-free culture medium. It has now been widely used to cultivate mammalian cells to prepare monoclonal antibodies, recombinant proteins, and viral antigens. So, what are the advantages and disadvantages of serum-free culture media? Let's take a look with the editor from Hengyuan!
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