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Research Information: Differences between Primary Cells, Cell Lines, and Cell Lines
Primary cells refer to cells obtained from tissues of the body through proteases or other methods and cultured in vitro, known as primary cells. It is generally believed that the first generation of cultured cells and cells passaged up to the 10th generation are collectively referred to as primary cell culture.
Differences between primary cells, cell lines, and cell lines
Cell line: After the first successful passage of the primary culture, it becomes a cell line composed of cell lineages that originally existed in the primary culture. If the passage cannot continue or the number of passages is limited, it can be called a limited cell line. If continuous cultivation is possible, it is called a continuous cell line. Cultivate for more than 50 generations and continue indefinitely.
Cell line: A culture obtained from a primary culture or cell line by selection or clone formation with special properties or markers is called a cell line. In other words, a cell line is a group of cells formed by the proliferation of single cells through single cell isolation or screening. The special properties or markers of the cell line must remain present throughout the entire culture period. If the passage cannot continue or the number of passages is limited, it is called a limited cell line; If it can be continuously passaged, it is called a continuous cell line. For human tumor cells, those that have been cultured in vitro for more than six months, grown stably, and continuously passaged can be called continuous strains or strains.
The current elective textbooks of the People's Education Society outline introduce cell lines and cell lines as follows: primary cultured cells are generally difficult to pass down after about 10 generations, and cell growth will stagnate, with most cells aging and dying. However, a very small number of cells are able to survive the crisis and continue to pass on, and these surviving cells can generally pass on to 40-50 generations, which are called cell lines. The genetic material of the cell line has not changed. After 50 generations of cell transmission, there will be a "crisis" that cannot continue. However, some cells have undergone changes in their genetic material and exhibit characteristics of carcinogenesis, making it possible for them to be passaged without restriction under culture conditions. These passaged cells are called cell lines.
The advantage of primary cells is that their characteristics and biological characteristics are close to their physiological state, but their disadvantage is that they are difficult to separate and have limited growth time. The advantage of cell lines or cell lines is that they can be passed through multiple generations or infinitely, making them easier to cultivate. However, the disadvantage is that as the number of culture generations increases, certain physiological traits of cells may be lost.
Based on the above comparison, many researchers choose primary cells for scientific experiments.
For more experimental information, please consult our sales representative. Shanghai Hengyuan Biotechnology Co., Ltd. has its own laboratory and a professional technical research and development team, focusing on the research and sales of various biological reagents, cells, serum, ELISA kits. The company provides free testing services for experiments and exquisite technical services. If you have any questions about experiments, please feel free to consult and provide professional one-on-one technical guidance.
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